The effect of pasteurisation and storage on aroma compounds in lager

Authors

DOI:

https://doi.org/10.58430/jib.v130i2.52

Keywords:

aroma compounds, bottled beer, unpasteurised beer, pale malt lager beer, storage

Abstract

Why was the work done: To investigate the impact of pasteurisation and storage in bottle on aroma compounds in pale lager beer.

How was the work done: Pale lager beer was produced at an industrial scale with 100% pilsner malt and a bottom fermenting yeast. Samples were taken of unpasteurised beer from bright beer tank, after flash pasteurisation and six months after packaging in amber glass bottles.

What are the main findings:  Post pasteurisation, a marked increase was found in the concentration of 2,3-pentanedione (50%) and diacetyl (33%),  presumably reflecting the decomposition by heat of precurser acetohydroxy acids.   There was also a marginal increase in dimethyl sulphide (6%) with little or no change in other aroma compounds. Storage for six months in bottle, also resulted in an increase in the level of 2,3-pentanedione,  diacetyl and dimethyl sulphide.   The linear (Pearson) correlation was > 0.8 for both dimethyl sulphide and diacetyl, and 2,3-pentanedione and diacetyl.  Accordingly, it was concluded that the levels in beer of dimethyl sulphide and 2,3-pentanedione are proportionally related to diacetyl.

Why is the work important: This work provides an insight into the effects on flavour and aroma of lager of flash pasteurisation and subsequent storage in bottle. The inter-relationship between aroma compounds in beer suggests that such synergies may undermine the sensory perception of threshold levels and identification of specific aromas.

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References

Analytica - EBC. 1998. European Brewery Convention. Fachverlag Hans Carl, Nürnberg.

Analytica-Microbiologica-EBC. 2005. European Brewery Convention. Fachverlag Hans Carl, Nürnberg.

Anness BJ, Bamforth CW. 1982. Dimethyl sulphide - A review. J Inst Brew 88:244-252. DOI: https://doi.org/10.1002/j.2050-0416.1982.tb04101.x

Cao L, Zhou G, Guo P, Li Y. 2011. Influence of pasteurising intensity on beer flavour stability. J Inst Brew 117:587-592. DOI: https://doi.org/10.1002/j.2050-0416.2011.tb00508.x

Clapperton JF. 1976. Ribes flavour in beer. J Inst Brew 82:175-176. DOI: https://doi.org/10.1002/j.2050-0416.1976.tb03746.x

Cui DY, Ge JL, Song YM, Feng PP, Lin LC, Guo LY, Zhang CY. 2021. Regulating the ratio of higher alcohols to esters by simultaneously overexpressing ATF1 and deleting BAT2 in brewer's yeast Saccharomyces pastorianus. Food Biosci 43:101231. DOI: https://doi.org/10.1016/j.fbio.2021.101231

De Gaetano G, Costanzo S, Di Castelnuovo A, Badimon L, Bejko D, Alkerwi A, Chiva-Blanch G, Estruch R, La Vecchia C, Panico S, Pounis G, Sofi F, Stranges S, Trevisan M, Ursini F, Cerletti C, Donati MB, Lacoviello L. 2016. Effects of moderate beer consumption on health and disease: A consensus document. Nutr Metab Cardiovasc Dis 26:443-467. DOI: https://doi.org/10.1016/j.numecd.2016.03.007

De Lima AC, Aceña L, Mestres M, Boqué R. 2023. Monitoring the evolution of the aroma profile of lager beer in aluminium cans and glass bottles during the natural ageing process by means of HS-SPME/GC-MS and multivariate analysis, Molecules 28:2807. DOI: https://doi.org/10.3390/molecules28062807

Dzialo MC, Park R, Steensels J, Lievens B, Verstrepen KJ. 2017. Physiology, ecology and industrial applications of aroma formation in yeast. FEMS Microbiol Rev 41:S95–S128. DOI: https://doi.org/10.1093/femsre/fux031

EBC Technology and Engineering Forum. 1995. Beer pasteurisation: Manual of Good Practice, Fachverlag Hans Carl, Nurnberg.

Ferreira IM, Guido LF. 2018. Impact of wort amino acid on beer flavour: a review. Fermentation 4:23. DOI: https://doi.org/10.3390/fermentation4020023

Gagula G, Mastanjević K, Mastanjević K, Krstanović V, Horvat D, Magdić D. 2000. The influence of packaging material on volatile compounds of pale lager beer. Food Packag Shelf Life 24:100496. DOI: https://doi.org/10.1016/j.fpsl.2020.100496

Gagula G, Šarić G, Rezić T, Horvat D, Magdić D. 2023. Changes in the physicochemical properties of pale lager beer during storage in different packaging Materials. J Am Soc Brew Chem 81:351-356. DOI: https://doi.org/10.1080/03610470.2022.2068318

Haukeli AD, Jacobsen T, Lie S. 1973. Regression analysis of some beer volatiles. Tech Q Master Brew Assoc Am 10:47-52.

Haukeli AD, Lie S. 1974. Formation and removal of acetoin during yeast fermentation. J Inst Brew 81:58-64. DOI: https://doi.org/10.1002/j.2050-0416.1975.tb03662.x

Holt S, Miks MH, de Carvalho BT, Foulquié-Moreno MR, Thevelein JM. 2019. The molecular biology of fruity and floral aromas in beer and other alcoholic beverages. FEMS Microbiol Rev 43:193-222. DOI: https://doi.org/10.1093/femsre/fuy041

Horn CS, Franke M, Blakemore FB, Stannek W. 1997. Modelling and simulation of pasteurisation and staling effects during tunnel pasteurisation of bottled beer, Food Bioprod Process 75:23-33. DOI: https://doi.org/10.1205/096030897531333

Hughes PS, Baxter ED. 2001. Beer: Quality, Safety and Nutritional Aspects. Royal Society of Chemistry. Cambridge, UK.

Humia BV, Santos KS, Barbosa, AM, Sawata M, Mendonça MDC, Padilha FF. 2019. Beer molecules and its sensory and biological properties: a review. Molecules 24:1568. DOI: https://doi.org/10.3390/molecules24081568

Jaskula-Goiris B, De Casmaecker B, De Rouck G, Aerts G, Paternoster A, Braet J, De Cooman L. 2019. Influence of transport and storage conditions on beer quality and stability. J Inst Brew 125:60-68. DOI: https://doi.org/10.1002/jib.535

Knatchbull FB, Slaughter JC. 1987. The effect of low CO2 pressures on the absorption of amino acids and production of flavour-active volatiles by yeast. J Inst Brew 93:420–424. DOI: https://doi.org/10.1002/j.2050-0416.1987.tb04530.x

Kobayashi M, Shimizu H, Shioya S. 2008. Beer volatile compounds and their application to low-malt beer fermentation. J Biosci Bioeng 106:317-323. DOI: https://doi.org/10.1263/jbb.106.317

Krogerus K, Gibson BR. 2013. Influence of valine and other amino acids on total diacetyl and 2,3-pentanedione levels during fermentation of brewer’s wort. Appl Microbiol Biotechnol 97:6919-6930. DOI: https://doi.org/10.1007/s00253-013-4955-1

Kucharczyk K, Żyła K, Tuszyński T. 2020. Volatile esters and fusel alcohol concentrations in beer optimized by modulation of main fermentation parameters in an Industrial plant. Processes 8:769. DOI: https://doi.org/10.3390/pr8070769

Lachenmeier DW, Haupt S, Schulz K. 2008. Defining maximum levels of higher alcohols in alcoholic beverages and surrogate alcohol products. Regul Toxicol Pharmacol 50:313-321. DOI: https://doi.org/10.1016/j.yrtph.2007.12.008

Lehnhardt F, Gastl M, Becker T. 2018. Forced into aging: Analytical prediction of the flavor-stability of lager beer. A review. Crit Rev Food Sci Nutr 59:2642–2653. DOI: https://doi.org/10.1080/10408398.2018.1462761

Liu C, Li Q, Niu C, Zheng F, Zhao Y. 2018. Simultaneous determination of diethylacetal and acetaldehyde during beer fermentation and storage process. J Sci Food Agric 98: 4733-4741 DOI: https://doi.org/10.1002/jsfa.9008

Lodolo EJ, Kock JL, Axcell BC, Brooks M. 2008. The yeast Saccharomyces cerevisiae - the main character in beer brewing. FEMS Yeast Res 8:1018-1036. DOI: https://doi.org/10.1111/j.1567-1364.2008.00433.x

Lerro M, Marotta G, Nazzaro C. 2020. Measuring consumers' preferences for craft beer attributes through Best-Worst Scaling. Agric Econ 8:1-13. DOI: https://doi.org/10.1186/s40100-019-0138-4

Marconi O, Rossi S, Galgano F, Sileoni V, Perretti G. 2016. Influence of yeast strain, priming solution and temperature on beer bottle conditioning. J Sci Food Agric 96:4106-4115. DOI: https://doi.org/10.1002/jsfa.7611

Mascia I, Fadda C, Karabín M, Dostálek P, Del Caro A. 2016. Aging of craft durum wheat beer fermented with sourdough yeasts. LWT Food Sci Technol 65:487-494. DOI: https://doi.org/10.1016/j.lwt.2015.08.026

Meilgaard MC. 1975. Flavour chemistry of beer. Part 1: flavour interaction between principal volatiles. Tech Q Master Brew Assoc Am 12:107-117.

MEBAK. 2013. Wort, beer and beer-based beverages. MEBAK, Freising-Weienstephan, Germany

Meilgarrd MC. 1982. Prediction of flavor differences between beers from their chemical composition. J Agric Food Chem 30:1009-1017. DOI: https://doi.org/10.1021/jf00114a002

Moreno-Llamas A, De la Cruz-Sánchez E. 2023. Moderate beer consumption is associated with good physical and mental health status and increased social support. Nutrients 15:1519. DOI: https://doi.org/10.3390/nu15061519

Moreira MTG, Pereira PR, Aquino A, Conte-Junior CA, Paschoalin VMF. 2022. Aldehyde accumulation in aged alcoholic beer: addressing acetaldehyde impacts on upper aerodigestive tract cancer risks. Int J Mol Sci 23:14147. DOI: https://doi.org/10.3390/ijms232214147

Olaniran AO, Hiralal, L, Mokoena, M. P., Pillay, B. 2017. Flavour-active volatile compounds in beer: production, regulation and control. J Inst Brew 123:13-23. DOI: https://doi.org/10.1002/jib.389

Pavlečić M, Tepalović D, Ivančić Šantek M, Rezić T, Šantek B. 2012. The effect of total oxygen concentration in the bottle on the beer quality during storage. Croat J Food Technol Biotech Nut 7:118-125.

Peppard, T. L. 1978. Dimethyltrisulphide, its mechanism of formation in hop oil and effect on beer flavor. J Inst Brew 84:337-340. DOI: https://doi.org/10.1002/j.2050-0416.1978.tb03903.x

Pires EJ, Teixeira JA, Brányik T, Vicente AA. 2014. Yeast: the soul of beer's aroma - a review of flavour-active esters and higher alcohols produced by the brewing yeast. Appl Microbiol Biotechnol 98:1937-1949. DOI: https://doi.org/10.1007/s00253-013-5470-0

Pires E, Brányik T. 2015. Biochemistry of Beer Fermentation. Springer, New York. DOI: https://doi.org/10.1007/978-3-319-15189-2

Preedy VR (Ed.) 2011. Beer in Health and Disease Prevention. Academic Press, London.

Rossi S, Sileoni V, Perretti G, Marconi O. 2014. Characterization of the volatile profiles of beer using headspace solid-phase microextraction and gas chromatography-mass spectrometry. J Sci Food Agric 94:919-928. DOI: https://doi.org/10.1002/jsfa.6336

Salanță LC, Coldea TE, Ignat MV, Pop CR, Tofană M, Mudura E, Borșa A, Pasqualone A, Zhao H. 2020. Non-alcoholic and craft beer production and challenges. Processes 8:1382. DOI: https://doi.org/10.3390/pr8111382

Scarlata CJ, Ebeler SE. 1999. Headspace solid-phase microextraction for the analysis of dimethyl sulphide in beer. J Agric Food Chem 47:2505-2508. DOI: https://doi.org/10.1021/jf990298g

Schneiderbanger H, Koob J, Poltinger, S, Jacob F, Hutzler M. 2016. Gene expression in wheat beer yeast strains and the synthesis of acetate esters. J Inst Brew 122:403-411. DOI: https://doi.org/10.1002/jib.337

Vanderhaegen B, Neven H, Verachtert H, Derdelinckx G. 2006. The chemistry of beer aging – a critical review. Food Chem 95:357-381. DOI: https://doi.org/10.1016/j.foodchem.2005.01.006

Vanderhaegen B, Neven H, Coghe S, Verstrepen KJ, Verachtert H, Derdelinckx G. 2003. Evolution of chemical and sensory properties during aging of top-fermented beer. J Agric Food Chem 51:6782-6790. DOI: https://doi.org/10.1021/jf034631z

Verstrepen KJ, Derdelinckx G, Dufour JP, Winderickx J, Thevelein JM, Pretorius IS, Delvaux FR. 2003. Flavor-active esters: adding fruitiness to beer. J Biosci Bioeng 96:110-118. DOI: https://doi.org/10.1263/jbb.96.110

Vesely P, Volgyi A, Lusk LT, Basarova G, Navarro A, Seabrooks J, Ryder D. 2004. Impact of esterase activity in aseptically packaged, unpasteurised beer. Tech Q Master Brew Assoc Am 41:293-297.

Vidal EE, de Morais MA Jr, Francois JM, de Billerbeck GM. 2015. Biosynthesis of higher alcohol flavour compounds by the yeast Saccharomyces cerevisiae: impact of oxygen availability and responses to glucose pulse in minimal growth medium with leucine as sole nitrogen source. Yeast 32:47–56. DOI: https://doi.org/10.1002/yea.3045

Wainwright T. 1973. Diacetyl - a review. Part 1 - analytical and biochemical considerations: Part II - brewing experience. J Inst Brew 79:451-470. DOI: https://doi.org/10.1002/j.2050-0416.1973.tb03567.x

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29-04-2024 — Updated on 29-04-2024

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Gagula , G., Đurđević-Milošević, D., Ncube , T., & Magdić, D. (2024). The effect of pasteurisation and storage on aroma compounds in lager . Journal of the Institute of Brewing, 130(2), 83–92. https://doi.org/10.58430/jib.v130i2.52