A continuous mashing system controlled by mean residence time
DOI:
https://doi.org/10.58430/jib.v129i1.7Keywords:
continuous mashing, continuous stirred tank reactor, mean residence time, fermentable sugarAbstract
Continuous processes offer more environmentally friendlier beer production compared to the batch production. However, the continuous production of mashing has not become state-of-the-art in the brewing industry. The controllability and flexibility of this process still has hurdles for practical implementation, but which are necessary to react to changing raw materials. Once overcome, a continuous mashing can be efficiently adapted to the raw materials. Both mean residence time and temperature were investigated as key parameters to influence the extract and fermentable sugar content of the wort. The continuous mashing process was implemented as continuous stirred tank reactor (CSTR) cascade consisting of mashing in (20°C), protein rest (50°C), β-amylase rest (62-64°C), saccharification rest (72°C) and mashing out (78°C). Two different temperature settings for the β-amylase rest were investigated with particular emphasis on fermentable sugars. Analysis of Variance (ANOVA) and a post-hoc analysis showed that the mean residence time and temperature settings were suitable control parameters for the fermentable sugars. In the experimental conditions, the most pronounced effect was with the β-amylase rest. These results broaden the understanding of heterogenous CSTR mashing systems about assembly and selection of process parameters
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References
Bamforth CW. 2003. Barley and malt starch in brewing: a general review. Tech Q Master Brew Assoc 40:89–97.
Bamforth CW. 2009. Current perspectives on the role of enzymes in brewing. J Cereal Sci 50:353–357.
Bancila D-M, Popescu D, Chiriac AI, Colbu S-C, Olteanu SC and Petrescu C. 2021. Chemical non-isothermal processes-stability study and optimal control. In 25th Int Conf Syst Theory, Control Comput, 18–24.
Bee Wah Y and Mohd Razali N. 2011. Power comparisons of Shapiro-Wilk, Kolmogorov-Smirnov, Lilliefors and Anderson-Darling tests. J Stat Model Anal 2:21–33.
Bellut K, Michel M, Zarnkow M, Hutzler M, Jacob F, De Schutter D, Daenen L, Lynch K, Zannini E and Arendt E. 2018. Application of non-Saccharomyces yeasts isolated from kombucha in the production of alcohol-free beer. Fermentation 4:66.
Brandam C, Meyer XM, Proth J, Strehaiano P and Pingaud H. 2003. An original kinetic model for the enzymatic hydrolysis of starch during mashing. Biochem Eng J 13:43–52.
Cai S, Yu G, Chen X, Huang Y, Jiang X, Zhang G and Jin X. 2013. Grain protein content variation and its association analysis in barley. BMC Plant Biol 13:35.
Colbu S-C, Hamidi F, Popescu D, Jerbi H and Bancila D-M. 2021. Stability analysis and control for non-isothermal CSTR reactors. In 9th Int Conf Syst Control, 504–509.
Cook AH and Davis AD. 1960. Process and apparatus for the preparation of worts. US Patent No. 2,961,316.
Dai F, Wang J, Zhang S, Xu Z and Zhang G. 2007. Genotypic and environmental variation in phytic acid content and its relation to protein content and malt quality in barley. Food Chem 105:606–611.
Durand GA, Corazza ML, Blanco AM and Corazza FC. 2009. Dynamic optimization of the mashing process. Food Control 20:1127–1140.
Evans DE, Collins H, Eglinton J and Wilhelmson A. 2005. Assessing the impact of the level of diastatic power enzymes and their thermostability on the hydrolysis of starch during wort production to predict malt fermentability. J Am Soc Brew Chem 63:185–198.
Evans DE and Fox GP. 2017. Comparison of diastatic power enzyme release and persistence during modified Institute of Brewing 65°C and Congress programmed mashes. J Am Soc Brew Chem 75:302–311.
Fox G. 2016. Infrared spectral analysis of sugar profiles of worts from varying grist to liquor ratios using infusion and ramping mash styles. J Inst Brew 122:437–445.
Fox GP, Staunton M, Agnew E and D’Arcy B. 2019. Effect of varying starch properties and mashing conditions on wort sugar profiles. J Inst Brew 125:412–421.
Gupta M, Abu-Ghannam N and Gallaghar E. 2010. Barley for brewing: characteristic changes during malting, brewing and applications of its by-products. Compr Rev Food Sci Food Saf 9:318–328.
Henson CA, Duke SH and Vinje MA. 2014. A Comparison of barley malt amylolytic enzyme thermostabilities and wort sugars produced during mashing. J Am Soc Brew Chem 72:51–65.
Hertel M and Sommer K. 2016. Wort boiling method and apparatus. US Patent No.9,434,917 B2.
Hudson JRH and Button AH. 1968. A novel pilot brewery. J Inst Brew 74:300–304.
Hui YH. 2007. Handbook of Food Products Manufacturing. Hoboken, NJ, USA: John Wiley & Sons, Inc.
Huppmann F. 1969. Plant for the continuous preparation of brewers’s mash and for the cooking of wort. US Patent No. 3,468,240.
Igbokwe PK, Nwabanne JT and Gadzama SW. 2015. Characterization of a 5 litre continuous stirred tank reactor. World J Eng Technol. 03:25–40.
Jones BL. 2005. Endoproteases of barley and malt. J Cereal Sci 42:139–156.
Jones BL and Marinac L. 2002. The effect of mashing on malt endoproteolytic activities. J Agric Food Chem 50:858–864.
Katsch L, Methner F-J and Schneider J. 2020. Kinetic studies of L-ascorbic acid degradation in fruit juices for the improvement of pasteurization plants. BrewSci 73:85–94.
Katsch L, Methner F-J and Schneider J. 2021. Kinetic studies of 5-(hydroxymethyl)-furfural formation and change of the absorption at 420 nm in fruit juices for the improvement of pasteurization plants. Int J Food Eng 17:703–713.
Kehse W and Jess U. 1974. Continuous production of beer wort from dried malt. US Patent No.3,834,296.
Kempfert NR. 2016. System and method for all-in-one wort preparation. U.S. Patent No.9,499,776 B2.
Koljonen T, Hämäläinen JJ, Sjöholm K and Pietilä K. 1995. A model for the prediction of fermentable sugar concentrations during mashing. J Food Eng 26:329–350.
Koljonen T, Kettunen A, Sjoholm K, Pietila K and Hamalainen JJ. 1992. Simulation of enzyme kinetics during malt mashing - a new parameter estimation method. In Proc 1992 First IEEE Conf Control Appl, 90–95.
Ma Y, Stewart DC, Eglinton JK, Logue SJ, Langridge P and Evans DE. 2000. Comparative enzyme kinetics of two allelic forms of barley (Hordeum vulgare L.) beta -amylase. J Cereal Sci 31:335–344.
MacGregor AW, Bazin SL, Macri LJ and Babb JC. 1999. Modelling the contribution of alpha-amylase, beta-amylase and limit dextrinase to starch degradation during mashing. J Cereal Sci 29:161–169.
Malar S and Thyagarajan T. 2009. Modelling of continuous stirred tank reactor using artificial intelligence techniques. Int J Simul Model 8:145–155.
Marcinkowska-Lesiak M, Zdanowska-Sąsiadek Z, Stelmasiak A, Damaziak K, Michalczuk M, Poławska E, Wyrwisz J and Wierzbicka A. 2016. Effect of packaging method and cold-storage time on chicken meat quality. CYTA - J Food 14:41–46.
Martin AD. 2000. Interpretation of residence time distribution data. Chem Eng Sci 55:5907–5917.
Martinez-Amezaga NMJ, Rovaletti MML, Benítez EI, Amezaga NMJM, Rovaletti MML and Benítez EI. 2018. Particle size distribution of polysaccharides in beer before the filtration process. Int J Food Res 5:13–19.
Moll M, Bastin MF and Peters B. 1976. Method of mashing for the production of wort and apparatus for the carrying out of this process. US Patent No.3,989,848.
Montanari L, Floridi S, Marconi O, Tironzelli M and Fantozzi P. 2005. Effect of mashing procedures on brewing. Eur Food Res Technol 221:175–179.
Mulder H. 2012. Continuous Method of Producing a Mash Extract. US Patent No.8,202,702 B2.
Mulder H and Snip OC. 2018. Method of producing a mash extract and an apparatus for performing such method. US Patent No.9,879,208 B2.
Muller R. 1991. The effects of mashing temperature and mash thickness on wort carbohydrate composition. J Inst Brew 97:85–92.
Osman AM. 2002. The advantages of using natural substrate-based methods in assessing the roles and synergistic and competitive interactions of barley malt starch-degrading enzymes. J Inst Brew 108:204–214.
Osmekhina E, Neubauer A, Klinzing K, Myllyharju J and Neubauer P. 2010. Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylase. Microb Cell Fact 9:1–11.
Quek WP, Yu W, Fox GP and Gilbert RG. 2019. Molecular structure-property relations controlling mashing performance of amylases as a function of barley grain size. Amylase 3:1–18.
Roussinova V and Kresta SM. 2008. Comparison of continuous blend time and residence time distribution models for a stirred tank. Ind Eng Chem Res 47:3532–3539.
De Ruyck H. 1997. Modelling of the residence time distribution in a twin screw extruder. J Food Eng 32:375–390.
Silhavy JF and Saginaw M. 1938. Method of and apparatus for producing wort or the like. US Patent No. 2,127,759.
Singh A and Sharma V. 2013. Concentration control of CSTR through fractional order PID controller by using soft techniques. In 2013 Fourth Int Conf Comput Commun Netw Technol, 1–6.
Steiner E, Gastl M and Becker T. 2011. Protein changes during malting and brewing with focus on haze and foam formation: a review. Eur Food Res Technol 232:191–204.
Stohle L and Wold S. 1989. Analysis of variance (ANOVA). Chemom Intell Lab Syst 6:259–272.
Strobl M. 2020. Continuous beer production. In New Adv Ferment Process. IntechOpen, 13.
Taskila S, Osmekhina E, Tuomola M, Ruuska J and Neubauer P. 2011. Modification of buffered peptone water for improved recovery of heat-injured Salmonella typhimurium. J Food Sci 76.
Toftgaard Pedersen A, de Carvalho TM, Sutherland E, Rehn G, Ashe R and Woodley JM. 2017. Characterization of a continuous agitated cell reactor for oxygen dependent biocatalysis. Biotechnol Bioeng 114:1222–1230.
Toson P, Doshi P and Jajcevic D. 2019. Explicit residence time distribution of a generalised cascade of continuous stirred tank reactors for a description of short recirculation time (bypassing). Processes 7:615.
Tsai DDW and Chen PH. 2013. Differentiation criteria study for continuous stirred tank reactor and plug flow reactor. Theor Found Chem Eng 47:750–757.
Watts PH, Ash ME and Philpotts GC. 1964. Method for preparing brewers’ wort. US Patent No. 3,128,189.
Wefing P, Conradi F, Trilling M, Neubauer P and Schneider J. 2020. Approach for modelling the extract formation in a continuous conducted ‘β-amylase rest’ as part of the production of beer mash with targeted sugar content. Biochem Eng J 164:107765.
Wijngaard HH and Arendt EK. 2006. Optimisation of a mashing program for 100% malted buckwheat. J Inst Brew 112:57–65.
Willaert RG and Baron G V. 2001. Wort boiling today-boiling systems with low thermal stress in combination with volatile stripping. Cerevisia 26:217–230.
de Winter JCF. 2013. Using the student’s t-test with extremely small sample sizes. Pract Assessment, Res Eval 18:1–12.
Yin C, Zhang G-P, Wang J-M and Chen J-X. 2002. Variation of beta-amylase activity in barley as affected by cultivar and environment and its relation to protein content and grain weight. J Cereal Sci 36:307–312.
Ziegler P. 1999. Cereal beta-amylases. J Cereal Sci 29:195–204.
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